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Federal government websites often end in. The site is secure. Preview improvements coming to the PMC website in October Learn More or Try it out now. When euthanizing an animal, it is important to confirm death before proceeding to necropsy or carcass storage. In maximum biocontainment labs ABSL-4 , common methods of confirmation, such as auscultation with a stethoscope or palpation of pulse, are not possible.
Our previous approach was to use a pulse oximeter to monitor for loss of signal and observe cessation of respiration. In many cases, we would lose signal from the pulse oximeter prior to administering euthanasia solution, so this reading could not be used to confirm death. As an improvement to this approach, we decided to try confirming euthanasia using Doppler flow detector since absence of pulsatile arterial flow should more accurately represent death than loss of a peripheral pulse oximetry signal.
To start, we used a portable Doppler device on sedated, noninfected nonhuman primates to verify accurate placement of the probe to hear a heartbeat. After practice outside of maximum containment, we used the Doppler device on moribund animals and also during end-of-study procedures on infected animals. While using the Doppler device simultaneously with pulse oximetry on all animals, we observed a longer duration of signal using the Doppler device.
We were also able to determine that a heartbeat was present after cessation of respiration. While the majority of these comparisons have been in non-moribund animals, we plan to gather more data on moribund animals as they present to quantify further this improvement. In conclusion, we were able to confirm euthanasia more confidently with the Doppler device under maximum biocontainment conditions compared with our previous method of confirmation.
