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Seminal microflora is crucial to male fertility. Dysbiosis—disturbance of quantitative ratios of individual bacteria or appearance of pathogenic species—rarely results in symptomatic disease. Inflammation results in decreased sperm production, lower motility, or morphological changes and, in the long term, can cause ejaculatory duct obstruction, leading to infertility.

Dysbiosis in both partners results in fertility problems, disorders in embryo implantation, or miscarriages. In addition, chronic inflammation of the male genitourinary system may accelerate the appearance of antisperm antibodies.

A comprehensive examination of seminal microflora can clarify the causes of infertility or prevent pathological conditions that affect seminal parameters. Seminal microflora as a direct impact on fertility problems as well as a decrease in the effectiveness of assisted reproduction methods, insemination, or in vitro procedures.

Semen is usually examined bacteriologically only when genitourinary infection is suspected and the results of standard physical and biochemical examination indicate it Dohle et al. Initially, it was believed that the urine and semen of a healthy man were free of bacteria or that bacteria are present only occasionally Tomaiuolo et al. Bacteriological examination of the semen was reduced to the classical cultivation of microorganisms in aerobic conditions and, rarely, in anaerobic conditions.