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In particular, a vector system comprising a first expression cassette encoding BCL2 associated X apoptosis regulator BAX and a hypermethylated second expression cassette encoding a secreted glutamic acid decarboxylase 65 sGAD55 are administered to the patient to induce a tolerogenic response. A promising class of immunotherapies utilize the natural cell death process, apoptosis [], which is a natural non-inflammatory tolerance-inducing pathway.

Antigen-presenting cells APCs , such as dendritic cells DCs , become tolerogenic after engulfing apoptotic cells; this enables the presentation of processed apoptotic cell autoantigens without co- stimulation to regulatory T cells Tregs for stimulation or to autoreactive memory effector T cells Teff for inactivation []. In particular, a vector system comprising a a first expression cassette encoding BCL2 associated X apoptosis regulator BAX ; and b a hypermethylated second expression cassette encoding a secreted form of glutamic acid decarboxylase 65 e.