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A genome sequence from a modern human skull over 45,000 years old from Zlatý kůň in Czechia

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We found that she belonged to a population that appears to have contributed genetically neither to later Europeans nor to Asians.

Only three genomes have been recovered from individuals that fall close in time to the settlement of Europe and Asia more than 40 thousand years ago ka 1 , 2. Archaeological investigations ascribed the stone and bone tools retrieved from the cave to the early Upper Palaeolithic. However, the artefacts in association with this individual could not be confidently assigned to any specific cultural technocomplex 6 , 8.

We therefore extracted the amino acid hydroxyproline from leftover collagen to attempt to date a compound-specific fraction from the bone However, we suspect this is also artificially young due to the presence of trace exogenous contaminants derived from animal glue, as supported by genetic analysis discussed below and in Supplementary Section 2. We therefore conclude that the hydroxyproline determination reflects a minimum age, with the true age likely to be much older.

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