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Knowledge of spontaneous aberrations, namely, of their frequency in non-irradiated cells is of paramount importance not only in cytogenetic research, but also in contemporary animal production.
To obtain metaphase chromosomes the conventional method of lymphocyte cultivation was used, albeit slightly modified and adapted to the examined animals and the laboratory conditions. In contemporary animal production genetics, in particular, population genetics plays an important role. Its development is closely linked to the introduction of regular check-ups of production properties of domestic animals. An ever-increasing consumption of food products of animal origin, above all, of meat and milk, and consequently, the issue of cost-effective food production, have impacted the genetic research in the sense of its orientation towards finding more advanced methods for objective assessment of the hereditary factors in production properties of domestic animals.
Cytogenetic research enables us to gain a better insight into a chromosome structure. This research is not only significant for its purely scientific merit but also for its application potential, and as such, it provides a backbone in contemporary animal production 1. In our environment there exist a number of physical, chemical and biological agents which have a harmful effect on living organisms. These agents are divided into endogenous agents deriving from the organism and being a common cause of a spontaneous mutation; in respect of their nature, they can be either genetic or non-genetic and exogenous genotoxic agents deriving from natural or artificial sources 2.
