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Federal government websites often end in. The site is secure. Preview improvements coming to the PMC website in October Learn More or Try it out now. Louis, St. Louis, Missouri, United States of America. Adenovirus serotype 5 Ad5 vectors are well suited for gene therapy.
However, tissue-selective transduction by systemically administered Ad5-based vectors is confounded by viral particle sequestration in the liver.
The obtained data demonstrate the utility of the Robo4 promoter in an Ad5 vector context. Substitution of the hypervariable region 7 HVR7 of the Ad5 hexon with HVR7 from Ad serotype 3 resulted in decreased liver tropism and dramatically altered biodistribution of gene expression.
The results of these studies suggest that the combination of liver detargeting using a genetic modification of hexon with an endothelium-specific transcriptional control element produces an additive effect in the improvement of Ad5 biodistribution. Currently, adenoviral Ad vectors are widely used as gene delivery vehicles.