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The current study intended to genetically analyze the full length genome of the HBoV-1 of recently detected Egyptian strains. Seven overlapping sets of primers were developed to amplify a nearly complete HBoV-1 genome from the clinical samples. Sequencing was conducted using the same sets of primers. HBoV-1 virus strains were genetically analysed based on the sequences of their complete genomes and the individual open reading frames. The new sets of primers successfully amplified the three tested strains.

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